City come to Burnley on the back of a five-match unbeaten run, including a mid-week 1-1 draw at Borussia Monchengladbach which sealed their place in the last 16 of the Champions League.
But Guardiola's side will have less than three full days to recover from that match in Germany before the lunch-time kick-off against the Clarets.
And despite Burnley going into the game on the back of a 4-0 beating at West Bromwich Albion on Monday night, former Barcelona legend Guardiola is wary of a positive reaction and the Clarets' impressive home form.
The City boss - whose side will go top of the table with a win before Liverpool and league leaders Chelsea kick-off later in the day - said: “I saw their games yesterday. I am really impressed. I think it will be the toughest for our regeneration for 12.30. It is a derby. It is many things.
“We cannot forget they beat Liverpool. They lost in the 93rd-minute against Arsenal. They draw in Old Trafford. What they do, they do really well, I am really impressed about their quality, their spirit.
“Of course they lost the last game 4-0 to West Brom. I can imagine what happened when a team loses 4-0.
"Their attention and focus is higher than before. I prefer teams when they win a lot of games. They didn’t win. In this moment, they are ready and prepared.
“In terms of intensity, I think they will be better. Their legs are faster and cleaner than ours, and it is so important for us because after we have one week to prepare the next round of matches, Chelsea, Leicester and Watford."
City captain Vincent Kompany will miss the trip to Burnley through injury and defender Pablo Zabaleta and striker Sergio Aguero both faces fitness checks.