A youthful Development Squad went down to a 4-0 defeat against Rochdale at Gawthorpe on Tuesday.
Coach Michael Jolley named an unexperienced side, in contrast to the one that won 5-0 at Aston Villa last week, with only Jason Gilchrist and Tom Anderson having featured in a match day squad.
And the Clarets were up against a Dale side that featured a number of senior players, including 32-year-old stand-in midfielder Michael Rose, who played a big part in their promotion from League Two last season.
The Clarets went behind early on when Dale opened the scoring following a slick move.
It wasn't until the 27th minute that Burnley had their first chance on goal. Gilchrist neatly laid-off to Jamie Frost before the winger saw his effort well saved.
Moments later Rochdale doubled their advantage after another smart move down the left resulted in another ball across goal that evaded everyone, leaving striker Calvin Andrew to smash home.
Gilchrist, who has been in prolific form since pre-season, came close to bringing the youngsters back into the game as he found himself through on goal, but was forced out wide by a smart piece of defending and his shot was blocked to safety.
The Greater Manchester side scored their third five minutes into the second half, despite a superb goal-line clearance from Anderson, who could do nothing about the follow up.
The Clarets grew into the game following the introductions of Ntumba Massanka, Bradley Jackson and Shaun Hobson, with striker Massanka seeing his effort tipped over.
Dale countered and broke before unleashing a venomous strike from 30-yards which required a fine save from Northern Ireland U-19 international Conor Mitchell to flick over for a corner.
But with six minutes remaining, Dale scored their fourth with a firm header from a corner.
Burnley went looking for a consolation, but there would be no reward as Gilchrist’s effort was well saved twice as the clock ran down.
Burnley: Mitchell, Richardson (Hobson), Anderson, Whitmore, Nugent, Galvin (Jackson 59), Daly, O'Neill (Wilson 75), Frost, Trialist (Massanka 46), Gilchrist.